Plankton Culture Manual Download


FAO FISHERIES TECHNICAL PAPER
361

Edited by
Patrick Lavens and Patrick Sorgeloos
Laboratory of Aquaculture and Artemia Reference Center
University of Ghent
Ghent, Belgium

Phytoplankton Identification Manual National Institute of Oceanography Disclaimer: The authors are responsible for the contents of this manual First Edition: March 2004 X.N. Verlencar Somshekar Desai National Institute of Oceanography Dona Paula, Goa - 403 004 Editors V.K. Dhargalkar B.S. Ingole National Institute of Oceanography. Apr 16, 2014  Alright here's a quick video to kinda update you on todays progress on the propagation room and a brief overview of my phytoplankton culturing setup, if you have any questions just comment and I will.

Food and Agriculture Organization of the United Nations

Pdf

Rome, 1996

The designations employed and the presentation of material in this publication do not imply the expression of any opinion whatsoever on the part of the Food and Agriculture Organization of the United Nations concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries.

M-44
ISBN 92-5-103934-8

All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying or otherwise, without the prior permission of the copyright owner. Applications for such permission, with a statement of the purpose and extent of the reproduction, should be addressed to the Director, Information Division, Food and Agriculture Organization of the United Nations, Viale delle Terme di Caracalla, 00100 Rome, Italy.

© FAO 1996

Table of Contents

2.1. Introduction
2.2. Major classes and genera of cultured algal species
2.3. Algal production

2.3.1.1. Culture medium/nutrients
2.3.1.2. Light
2.3.1.3. pH
2.3.1.4. Aeration/mixing
2.3.1.5. Temperature
2.3.1.6. Salinity

2.3.2. Growth dynamics
2.3.3. Isolating/obtaining and maintaining of cultures
2.3.4. Sources of contamination and water treatment
2.3.5. Algal culture techniques

2.3.5.1. Batch culture
2.3.5.2. Continuous culture
2.3.5.3. Semi-continuous culture

2.3.6. Algal production in outdoor ponds
2.3.7. Culture of sessile micro-algae
2.3.8. Quantifying algal biomass
2.3.9. Harvesting and preserving micro-algae
2.3.10. Algal production cost

2.4. Nutritional value of micro-algae
2.5. Use of micro-algae in aquaculture

2.5.1. Bivalve molluscs
2.5.2. Penaeid shrimp
2.5.3. Marine fish

2.6.1. Preserved algae
2.6.2. Micro-encapsulated diets
2.6.3. Yeast-based diets

2.7. Literature of interest
2.8. Worksheets

Worksheet 2.1.: Isolation of pure algal strains by the agar plating technique
Worksheet 2.2.: Determination of cell concentrations using haematocytometer according to Fuchs-Rosenthal and Burker.
Worksheet 2.3.: Cellular dry weight estimation of micro-algae.

3.1. Introduction
3.2. Morphology
3.3. Biology and life history
3.4. Strain differences
3.5. General culture conditions

3.5.1.1. Salinity
3.5.1.2. Temperature
3.5.1.3. Dissolved oxygen
3.5.1.4. pH
3.5.1.5. Ammonia (NH3)
3.5.1.6. Bacteria
3.5.1.7. Ciliates

3.5.2. Freshwater rotifers
3.5.3. Culture procedures

Plankton Culture Manual Download

3.5.3.1. Stock culture of rotifers
3.5.3.2. Upscaling of stock cultures to starter cultures
3.5.3.3. Mass production on algae
3.5.3.4. Mass production on algae and yeast
3.5.3.5. Mass culture on yeast
3.5.3.6. Mass culture on formulated diets
3.5.3.7. High density rearing

3.6.1.1. Algae
3.6.1.2. Formulated feeds
3.6.1.3. Oil emulsions

3.6.2. Techniques for vitamin C enrichment
3.6.3. Techniques for protein enrichment
3.6.4. Harvesting/concentration and cold storage of rotifers

3.7. Production and use of resting eggs
3.8. Literature of interest
3.9 Worksheets

4.1.1. Introduction
4.1.2. Biology and ecology of Artemia

4.1.2.1. Morphology and life cycle
4.1.2.2. Ecology and natural distribution
4.1.2.3. Taxonomy
4.1.2.4. Strain-specific characteristics

4.2.1.1. Cyst morphology
4.2.1.2. Physiology of the hatching process
4.2.1.3. Effect of environmental conditions on cyst metabolism
4.2.1.4. Diapause

4.2.2. Disinfection procedures
4.2.3 Decapsulation
4.2.4. Direct use of decapsulated cysts
4.2.5. Hatching

4.2.5.1. Hatching conditions and equipment
4.2.5.2. Hatching quality and evaluation

4.2.6. Literature of interest
4.2.7. Worksheets

Worksheet 4.2.1.: Procedure for estimating water content of Artemia cysts
Worksheet 4.2.2.: Specific diapause termination techniques
Worksheet 4.2.3.: Disinfection of Artemia cysts with liquid bleach
Worksheet 4.2.4.: Procedures for the decapsulation of Artemia cysts
Worksheet 4.2.5.: Titrimetric method for the determination of active chlorine in hypochlorite solutions
Worksheet 4.2.6.: Artemia hatching
Worksheet 4.2.7.: Determination of hatching percentage, hatching efficiency and hatching rate

4.3.1. Harvesting and distribution
4.3.2. Cold storage
4.3.3. Nutritional quality
4.3.4. Enrichment with nutrients
4.3.5. Enrichment for disease control
4.3.6. Applications of Artemia for feeding different species

4.3.6.1. Penaeid shrimp
4.3.6.2. Freshwater prawn
4.3.6.3. Marine fish
4.3.6.4. Freshwater fish
4.3.6.5. Aquarium fish

4.3.7. Literature of interest
4.3.8. Worksheets

4.4.1. Nutritional properties of ongrown Artemia
4.4.2. Tank production

4.4.2.1. Advantages of tank production and tank produced biomass
4.4.2.2. Physico-chemical conditions
4.4.2.3. Artemia
4.4.2.4. Feeding
4.4.2.5. Infrastructure
4.4.2.6. Culture techniques
4.4.2.7. Enrichment of ongrown Artemia
4.4.2.8. Control of infections
4.4.2.9. Harvesting and processing techniques
4.4.2.10. Production figures and production costs

4.4.3. Literature of interest
4.4.4. Worksheets

4.5.1.1. Natural lakes
4.5.1.2. Permanent solar salt operations
4.5.1.3. Seasonal units

4.5.2.1. Climatology
4.5.2.2. Topography
4.5.2.3. Soil conditions

4.5.3.1. Large permanent salt operations
4.5.3.2. Small pond systems

4.5.4.1. Liming
4.5.4.2. Predator control
4.5.4.3. Fertilization

4.5.5.1. Artemia strain selection
4.5.5.2. Inoculation procedures

4.5.6.1. Monitoring the Artemia population
4.5.6.2. Abiotic parameters influencing Artemia populations
4.5.6.3. Biotic factors influencing Artemia populations

4.5.7.1. Artemia biomass harvesting and processing
4.5.7.2. Artemia cyst harvesting and processing

4.5.8. Literature of interest
4.5.9. Worksheets

Worksheet 4.5.1.: Pond improvements and harvesting procedures
Worksheet 4.5.2.: Procedures for the brine processing step
Worksheet 4.5.3.: Procedures for the freshwater processing step

5.1.1. Introduction
5.1.2. Collection from the wild
5.1.3. Collection techniques

5.1.3.1. Plankton nets
5.1.3.2. Trawl nets
5.1.3.3. Baleen harvesting system
5.1.3.4. Flow-through harvesting
5.1.3.5. Plankton light trapping

5.1.4. Zooplankton grading
5.1.5. Transport and storage of collected zooplankton

5.2.1. Introduction
5.2.2. Life cycle
5.2.3. Biometrics
5.2.4. Nutritional quality
5.2.5. Culture techniques

5.2.5.1. Calanoids
5.2.5.2. Harpacticoids

5.2.6. Use of resting eggs
5.2.7. Applications in larviculture

5.3.1. Introduction
5.3.2. Types of mesocosms

5.3.2.1. Pold system (2-60 m³)
5.3.2.2. Bag system (50-200 m³)
5.3.2.3. Pond system
5.3.2.4. Tank system

5.3.3. Mesocosm protocol
5.3.4. Comparison to intensive methods

6.1.1. Biology and life cycle of Daphnia
6.1.2. Nutritional value of Daphnia
6.1.3. Feeding and nutrition of Daphnia
6.1.4. Mass culture of Daphnia

6.1.4.1. General procedure for tank culture
6.1.4.2. Detrital system
6.1.4.3. Autotrophic system
6.1.4.4. General procedure for pond culture
6.1.4.5. Contamination

6.1.5. Production and use of resting eggs
6.1.6. Use of Moina

6.2. Nematodes
6.3. Trochophora larvae

6.3.1. Introduction
6.3.2. Production of trochophora larvae

6.3.2.1. Mussel larvae
6.3.2.2. Pacific oyster and Manila clam larvae

6.3.3. Quality control of the produced trochophora larvae
6.3.4. Cryopreservation

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